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NorthernLights™ Fluorescent Secondary Antibodies & Streptavidin Conjugates

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NorthernLights™ fluorescent secondary antibodies and streptavidin conjugates are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy (Figure 1)....

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Technical Note: Using NorthernLights Fluorescent Secondary Antibodies for Neuronal Tracing

Article

Immunofluorescence histochemistry is a powerful tool frequently used in neuroscience for qualitative and quantitative morphological and anatomical studies. Because samples are subjected to prolonged examination under conventional fluorescence microscopes and multiple laser-scanning confocal microscopes, it is critical for fluor­escent tags to have a low susceptibility to photo­bleaching....

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Targeted Gene Transfer with Fluorokines

Article

An inherent difficulty in the field of gene therapy is the delivery of the gene of interest to a specific target cell population. The ideal system should be both efficient and accurate.1 The use of retroviral vectors has proved both inefficient and non-specific in its quest to deliver genes to targets such as hematopoietic progenitor cell lines.2 A more efficient gene transfer system has been...

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Cell & Tissue Staining Kits

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Get the most out of your primary antibodies! Designed to optimize staining on cryostat, paraffin-embedded, and free-floating tissue sections, these kits provide all the reagents required to maximize your IHC experiments. Key Benefits Ready to use High Sensitivity Streptavidin (HSS) increases detection sensitivity Suitable for tissue sections & cytospin preparations Pre-diluted...

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NorthernLights™ Fluorescent Secondary Antibodies

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With more than 6000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your total source for immunofluorescence reagents. NorthernLights Antibody/Label Catalog # Abs/Em Maxima Laser (Ex) Comparable Fluorochromes NL-493 anti-Rabbit IgG NL-493 anti-Mouse IgG NL-493 anti-Goat IgG NL-493...

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Use of Proteome Profiler Arrays with LI-COR Detection

Technical Resource

R&D Systems’ Proteome Profiler arrays are rapid, sensitive, and economical multiplex arrays for the simultaneous detection of many proteins in a single sample. Originally developed for chemiluminescent detection, many Proteome Profiler arrays are amenable to near-infrared (NIR) fluorescence detection using the LI-COR Odyssey® Infrared Imaging System. To achieve this adaptation, the...

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Quantikine® HS Colorimetric Sandwich ELISA Kits, High Sensitivity ELISAs

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Complete Listing of Quantikine HS ELISA Kits Quantikine High Sensitivity ELISA Kits are complete assays generally used when very low levels of the target protein are expected. These kits utilize distinct protocols to achieve their impressive levels of analyte detection. Features Detect femtogram levels of protein Complete ready-to-use kits Exhaustively tested for superior quality...

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Detection & Visualization of Antibody Binding

Protocols

Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against...

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Cell and Tissue Staining Kits

Article

Figure 1. eNOS detection in human paraffin-embedded placenta tissue section using R&D Systems’ goat anti-human eNOS antibody (Catalog # AF950). Chromogenic detection was done using R&D Systems’ Cell and Tissue anti-goat AEC Staining Kit (Catalog # CTS009). Tissue was counterstained with Hematoxylin. Figure 2. Sonic Hedgehog (Shh) labeling in rat cerebellum was done using R&D...

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Flow Cytometry Supplemental Reagents

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Antibody Isotype Controls A wide selection of species-specific IgG isotype controls conjugated to a variety of labels. Secondary Antibodies We offer a range of secondary antibodies for flow cytometry, immunohistochemistry, and Western blotting. Staining Reagents for Flow Cytometry Flow Cytometry Staining Buffer (1X) (Catalog # FC001) For immunofluorescent staining of...

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