Showing 1-10 out of 91 for "phospho"
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Intracellular signal transduction factors, such as the MAP Kinases, have become important drug targets and experimental biomarkers. Because the activity of these factors is regulated by phosphorylation events, accurate detection of the level of phosphorylated target proteins is paramount to
IHC-Images
p53 phosphorylated at S46 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Phospho-p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1489) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell &
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Mouse Phospho-RTK Array Kit Ordering Information Introduction R&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for
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Human Phospho-RTK Array Kit Ordering Information Introduction R&D Systems™ Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001B) is a screening tool designed to simultaneously detect the relative phosphorylation of 49 different RTKs. This kit eliminates the need for
Article
R&D Systems now offers Phospho-Tyrosine antibodies suitable for all applications typically used to analyze protein tyrosine phosphorylation, including Western blotting, immunoprecipitation, immunohistochemistry, and ELISA. Detection of Tyrosine-Phosphorylated Proteins by Western Blotting
IHC-Images
Histone H2AX phosphorylated at S139 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Pan-specific phospho-Histone H2AX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL overnight at 4 °C. Before incubation with the primary
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Our new Human Phospho-Ubiquitin (S65) Antibody detects mono- and poly-Ubiquitin chains that are phosphorylated on serine 65 (see data below). It has no cross-reactivity with non-phosphorylated Ubiquitin, Ubiquitin phosphorylated on serine 57 or tyrosine 59, or phosphorylated Parkin. Start exploring
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Human Phospho-MAPK Array Kit Ordering Information Introduction The Human Phospho-Mitogen-activated Protein Kinase (MAPK) Antibody Array (Catalog # ARY002B) is an economical tool used to simultaneously detect the relative phosphorylation of 24 kinases without performing numerous immunoprecipitation
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Human Phospho-Kinase Antibody Array Ordering Information Introduction Analyzing the phosphorylation profiles of kinases and their protein substrates is essential for understanding how cells recognize and respond to changes in their environment. The Human Phospho-Kinase Array (Catalog # ARY003B) is
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Can other lysis buffers be used? Can a non-film based image acquisition system, such as a cooled CCD camera, be used? Can the array be stripped and reused? What exposure times should be used for chemiluminescent detection with X-ray film? Can the sensitivity be increased by altering the Detection
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