R&D Systems is pleased to offer a new screening tool to identify modulators of neural progenitor cell proliferation and differentiation. The 96-well format provides an easy method to screen for toxins, drugs, growth factors, and other substances that may affect neural stem cells. The Neural Toxicity Assay Kit (Catalog # SC014) can be used to determine the effects on both proliferation and differentiation; either adverse effects as with a toxin or increased rates of proliferation and differentiation resulting from a stimulatory agent.
Effect of Ethanol (EtOH) on neuronal differentiation of neural progenitor cells. EtOH has been shown to reduce levels of neuronal differentiation of neural progenitor cells. Rat cortical stem cells (Catalog # NSC001) were differentiated in the presence of the indicated amounts of EtOH. Levels of neuronal differentiation were determined using the Neural Toxicity Assay Kit (Catalog # SC014) as assessed by beta-III Tubulin levels. Cells were stained with DAPI as a relative measure of total cell number. Constant levels of DAPI staining indicate that decreases in beta-III Tubulin levels are the result of reduced differentiation rather than cell death.