14‑3‑3 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish
  • Specificity
    This antibody is specific for the 29 kDa 14-3-3 in Western blots of rat hippocampal lysates.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    14-3-3
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of 14-3-3 by Western Blot. Western blot of rat brain hippocampus lysate showing immunolabeling of the approximately 29 kDa 14-3-3 protein.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Background: 14-3-3
14-3-3 is a general name given to seven ubiquitously expressed, 28 kDa proteins that are the products of related, but separate genes. They are considered isoforms and are named eta ( eta ), gamma ( gamma ), delta/zeta (δ/ zeta ), alpha/beta ( alpha / beta ), theta/tau ( theta / tau ), sigma ( sigma ) and epsilon ( epsilon ). The first molecule was named for its 14th DEAE elution fraction and 3.3 migration pattern on gel electrophoresis. All seven molecules range from 245-255 amino acids in length and exhibit nine alpha -helices arranged in an antiparallel fashion. Overall amino acid sequence identity is approximately 49%, with most identity limited to six blocks of six or more amino acids. The invariant amino acids create a negatively-charged channel that is common to all isoforms. 14-3-3 proteins are known to bind over 200 client (mostly phosphorylated) proteins. The 14-3-3s are highly versatile, inhibiting, activating, linking and transporting a myriad of unrelated targets. They participate in all cellular processes. 14-3-3 proteins are both homodimers and heterodimers and also exist as monomers. Dimeric 14-3-3 generally binds to phosphorylated targets, generating some type of response. Monomeric 14-3-3 also appears to bind to the same targets, but without generating a response. The transition from dimer to monomer is controlled by phosphorylation, particularly by SKD1. The favored site for this phosphorylation is S59 on the eta and gamma isoforms, S58 on the zeta/delta isoform, and S60 on the alpha/beta isoform; tau, sigma and epsilon do not contain favorable motifs for phosphorylation.
  • References:
    1. Aitken, A. (2006) Semin. Cancer Biol. 16:162.
    2. Woodcock, J.M. et al. (2003) J. Biol. Chem. 278:36323.
    3. Muslin, A.J. et al. (1996) Cell 84:889.
    4. Hamaguchi, A. et al. (2003) Biochem. Biophys. Res. Commun. 307:589.
    5. Aitken, A. et al. (2002) Biochem. Soc. Trans. 30:351.
  • Alternate Names:
    14-3-3 protein tau; 14-3-3 protein T-cell; 14-3-3 protein theta; 1433; 14-3-3; 14-3-3,1C5; HS1; Protein HS1; protein tau; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 40  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 21  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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