African Swine Fever Virus p54 Antibody (GT853) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13671
![Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]](https://resources.rndsystems.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT853-Western-Blot-NBP3-13671-img0006.jpg "Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]")
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Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT853
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.
Reactivity Notes
African swine fever virus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for African Swine Fever Virus p54 Antibody (GT853) - Azide and BSA Free
Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]
Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of African Swine Fever Virus p54 protein (0.2 ug/ml) by lateral flow assay. Capture : Biotin-labeled African Swine Fever Virus p54 antibody [GT1075] (3.32 ug/ml)._x000B_ Detection : FITC-labeled African Swine Fever Virus p54 antibody [GT853] (3.32 ug/ml).![Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]](https://resources.rndsystems.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT853-Immunocytochemistry-Immunofluorescence-NBP3-13671-img0002.jpg "Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]")
Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]
Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]](https://resources.rndsystems.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT853-Immunohistochemistry-Paraffin-NBP3-13671-img0003.jpg "Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]")
Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]
Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - African Swine Fever Virus p54 antibody [GT853] detects African Swine Fever Virus p54 protein at cytoplasm by immunohistochemical analysis. Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]](https://resources.rndsystems.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT853-Western-Blot-NBP3-13671-img0001.jpg "Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]")
Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671]
Western Blot: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: HRP-conjugated African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) (1 ug/mL) Please notice that NBP3-13672 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13671. Please contact us for custom HRP-conjugated antibody.
Sandwich ELISA: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Sandwich ELISA detection of recombinant African Swine Fever Virus p54 using antibodies as below. Capture: African Swine Fever Virus p54 antibody [GT853] (NBP3-13671) (5 ug/mL) Detection: African Swine Fever Virus p54 antibody (NBP3-13435) (1 ug/mL).
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853])
Detection: African Swine Fever Virus p54 antibody
Samples (40 ng) :
1. African Swine Fever Virus p54 (ECD) protein
2. Lysis buffer
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] -
Lateral Flow Assay: African Swine Fever Virus p54 Antibody (GT853) [NBP3-13671] - Detection of HEK293 expressed, African Swine Fever Virus p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.Capture: African Swine Fever Virus p54 antibody (NBP3-13671 [GT853])
Detection: African Swine Fever Virus p54 antibody
Samples (40 ng) :
1. African Swine Fever Virus p54 (ECD) protein
2. Lysis buffer
Applications for African Swine Fever Virus p54 Antibody (GT853) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Lateral Flow Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: African Swine Fever Virus p54
Alternate Names
ASFV p54, Ba71V-126, E183L, Envelope protein p54, Structural protein p54
Gene Symbol
E183L
Additional African Swine Fever Virus p54 Products
Product Documents for African Swine Fever Virus p54 Antibody (GT853) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for African Swine Fever Virus p54 Antibody (GT853) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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