Benzonase® Nuclease/NucA Antibody

Catalog #: MAB100632 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB100632-100
MAB100632-SP
Detection of Benzonase® Nuclease/NucA by Western Blot.
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Benzonase® Nuclease/NucA Antibody Summary

Specificity
Detects Benzonase® Nuclease/NucA in direct ELISA.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2404D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived S. marcescens recombinant Benzonase® Nuclease/NucA
Met1-Asn266
Accession # P13717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rabbit Anti-Benzonase® Nuclease/NucA Monoclonal Antibody (Catalog # MAB10063).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Benzonase® Nuclease/NucA by Western Blot. View Larger

Detection of Benzonase® Nuclease/NucA by Western Blot. Western blot shows recombinant Benzonase® Nuclease/NucA protein. PVDF membrane was probed with 2 µg/mL of Rabbit Anti- Benzonase® Nuclease/NucA Monoclonal Antibody (Catalog # MAB100632) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Benzonase® Nuclease/NucA at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Benzonase® Nuclease/NucA by Simple Western<sup>TM</sup>. View Larger

Detection of Benzonase® Nuclease/NucA by Simple WesternTM. Simple Western lane view shows recombinant Benzonase® Nuclease/NucA protein, loaded at 0.2 mg/mL. A specific band was detected for Benzonase® Nuclease/NucA at approximately 35 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Benzonase® Nuclease/NucA Monoclonal Antibody (Catalog # MAB100632). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Benzonase® Nuclease/NucA ELISA Standard Curve. Recombinant Benzonase® Nuclease/NucA protein was serially diluted 2-fold and captured by Rabbit Anti-Benzonase® Nuclease/NucA Monoclonal Antibody (Catalog # MAB10063) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Benzonase® Nuclease/NucA Monoclonal Antibody (Catalog # MAB100632) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Benzonase® Nuclease/NucA

Long Name
Benzonase Nuclease
Entrez Gene IDs
23387471 (Bacterial)
Alternate Names
Benzonase® Nuclease; DcD

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