Intracellular Staining by Flow Cytometry
|Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry. Eight-day differentiated Rat cortical stem cells were stained with Mouse Anti-beta ‑III Tubulin PerCP‑conjugated Monoclonal Antibody (Catalog # IC1195C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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