Bovine IFN-gamma PE-conjugated Antibody

IC2300P has been discontinued. View all IFN-gamma products.
  
  • Species Reactivity
    Bovine
  • Specificity
    Detects bovine IFN-gamma in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with IFN-gamma from equine, canine, or feline systems is observed and no cross-reactivity with human, cotton rat, mouse, porcine, or rat IFN-gamma is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 345025
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant bovine IFN-gamma
    Gln24-Thr166
    Accession # NP_776511
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IFN‑ gamma in Bovine PBMCs by Flow Cytometry. Bovine peripheral blood mononuclear cells were stained with Rat Anti-Bovine IFN‑ gamma PE‑conjugated Monoclonal Antibody (Catalog # IC2300P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-gamma
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells including monocytes, NK cells, gamma δ T cells, CD8+ Tcells, multiple T cell subsets (inlcuding Th1, CCR7- TEM and CD103+CD69+ TRM cells) plus proinflammatory FoxP3 regulatory T cells (6-12). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 13, 14). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (15, 16). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). Finally, IFN-gamma regulates blood cell production, particularly during immune challenge. In particular, IFN-gamma appears to promote monocyte production while depressing neutrophil, B cell and eosinophil production (17).
  • References:
    1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
    2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
    3. Cerretti, D.P. et al. (1986) J. Immunol. 136:4561.
    4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
    5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
    6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
    7. Yamaguchi, R. et al. (2015) Blood Cells Mol. Dis. 55:127.
    8. Paolini, R. et al. (2015) Cytokine Growth Factor Rev. 26:113.
    9. Hou, L. et al. (2015) Int. Immunopharmacol. 28:887.
    10. de Graujo-Souza, P.S. et al. (2015) J. Immunol Res. 2015:849573.
    11. Greginat, J. et al. (2014) Front. Immunol. 5:630.
    12. Padiyan, P. and J. Zhu (2015) Cytokine 76:13.
    13. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
    14. Zhu, L. et al. (2014) Int. Rev. Immunol. 34:82.
    15. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
    16. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
    17. de Bruin, A.M. et al. (2014) Blood 124:2479.
  • Long Name:
    Interferon gamma
  • Entrez Gene IDs:
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
  • Alternate Names:
    IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Related Research Areas

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