C. botulinum BoNT-E Heavy Chain Antibody AF7135: R&D Systems

C. botulinum BoNT-E Heavy Chain Antibody

  
  • Species Reactivity
    C. botulinum
  • Specificity
    Detects C. botulinum BoNT-E Heavy Chain in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross-reactivity with BoNT-G/HCR and BoNT-A/HCR is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant C. botulinum BoNT-E Heavy Chain
    Lys845-Lys1251
    Accession # P30995
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of C. botulinum BoNT-E Heavy Chain by Western Blot. Western blot shows recombinant C. botulinum BoNT-E Heavy Chain (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-C. botulinum BoNT-E Heavy Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7135) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BoNT-E Heavy Chain at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BoNT-E Heavy Chain

BoNT/E HCR (Botulinum neurotoxin serotype E heavy chain receptor) is a 105 kDa component of BoNT/E, a member of the peptidase M27 family of molecules. BoNT/E is the product of Clostridium botulinum, and inhibits neurotransmitter release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of membrane-associated SNAP25, thus blocking synaptic vesicle fusion with the presynaptic membrane. The 155 kDa BoNT/E precursor is 1251 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage by host proteases, it assumes a mature form that contains a 50 kDa disulfide-linked 422 aa light chain/enzyme N-terminus, and a 105 kDa, 829 aa heavy chain/receptor-binding C-terminus. The heavy chain has two components, a 327 aa translocation domain (aa 518-840), and a 408 aa cell membrane SV2:ganglioside binding region (aa 844-1250). The heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. The E heavy chain shares less that 50% aa identity with the BoNT/A heavy chain.

  • Long Name:
    Botulinum Neurotoxin Type E Heavy Chain
  • Alternate Names:
    BoNTE Heavy Chain; BoNT-E Heavy Chain; Bontoxilysin-E
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used C. botulinum BoNT-E Heavy Chain Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information