Canine CD34 APC-conjugated Antibody

Discontinued Product

FAB3346A has been discontinued.
View all CD34 products.
Canine CD34 APC-conjugated Antibody in Data
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Canine CD34 APC-conjugated Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine CD34 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1H6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD34 antibody in CLL-1390 Canine Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 APC-conjugated Monoclonal Antibody (Catalog # FAB3346A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

CD34 is a 100 kDa highly glycosylated member of the CD34 family of molecules. In dog, it is a 366 amino acid (aa) type I transmembrane protein that contains a 271 aa extracellular domain (ECD). Although originally identified on the surface of hematopoietic stem cells and vascular endothelial progenitors, it is now known to be expressed by highly divergent cell types such as telocytes/ICLC (cardiac support cells), cytotoxic NK lineage restricted progenitors, muscle satellite cells, corneal keratocytes (fibroblasts) and dendritic cell progenitors (with the exception of pre-conventional DCs). Little is known about CD34 ligands, save for L-Selectin. Canine CD34 ECD shares 60% aa sequence identity with human CD34 ECD.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

Product Datasheets

FAQs

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