Canine CD34 Fluorescein-conjugated Antibody

    
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  • Species Reactivity
    Canine
  • Specificity
    Detects canine CD34 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 1H6
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34
    Accession # Q28270
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry. CLL‑1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3346F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD34
CD34 is a 100-125 kDa highly glycosylated member of the CD34 family of molecules. In canine, mature CD34 is a 356 amino acid (aa) type I transmembrane protein that contains a 261 aa extracellular domain (ECD). Although originally identified on the surface of hematopoietic stem cells and vascular endothelial progenitors, it is now known to be expressed by highly divergent cell types such as telocytes/ICLC (cardiac support cells), cytotoxic NK lineage restricted progenitors, muscle satellite cells, corneal keratocytes (fibroblasts) and dendritic cell progenitors (with the exception of pre-conventional DCs). Little is known about CD34 ligands, save for L-Selectin. Canine CD34 ECD shares 60% and 56% aa sequence identity with human and mouse CD34 ECD, respectively.
  • Entrez Gene IDs:
    947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
  • Alternate Names:
    CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 15  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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