Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.
CLL‑1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3346F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD34 is a 100-125 kDa highly glycosylated member of the CD34 family of molecules. In canine, mature CD34 is a 356 amino acid (aa) type I transmembrane protein that contains a 261 aa extracellular domain (ECD). Although originally identified on the surface of hematopoietic stem cells and vascular endothelial progenitors, it is now known to be expressed by highly divergent cell types such as telocytes/ICLC (cardiac support cells), cytotoxic NK lineage restricted progenitors, muscle satellite cells, corneal keratocytes (fibroblasts) and dendritic cell progenitors (with the exception of pre-conventional DCs). Little is known about CD34 ligands, save for L-Selectin. Canine CD34 ECD shares 60% and 56% aa sequence identity with human and mouse CD34 ECD, respectively.
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