Cloudz™ Cell Activation Kits

A reliable and scalable cell expansion platform is critical for a successful growing process. Research-and GMP-grade Cloudz kits, as well as custom options, are available to support consistent and seamless process translations. For our complete immune cell therapy manufacturing solution, visit ScaleReady.

Human T Cell Activation Kits

The Cloudz Human T Cell Activation Kits support the expansion of human T cells and the stimulation of human peripheral blood lymphocytes. The CD3 and CD28 antibodies on these Cloudz reagents were selected to optimally activate and expand CD4⁺ and CD8⁺ T cells. Activation of T cells with Cloudz microspheres enables efficient cell expansion while maintaining a high population of naïve-like memory cells. This kit is available in both research use only and GMP grades.  

Human Regulatory T Cells (Treg) Expansion Kits

Regulatory T cells, also known as suppressor cells, are a subset of CD4⁺ T cells that maintain immune homeostasis by suppressing immune responses and controlling autoimmune recognition. The Cloudz Human Treg Expansion Kits provides robust expansion of FoxP3⁺ human Treg cells from a population of CD4⁺ T cells.

Human Natural Killer (NK) Cell Expansion Kit

Natural killer cells are innate lymphoid cells with intrinsic cytotoxic and cytokine producing capabilities. These Cloudz kits provide a feeder-free expansion protocol that selectively expands NK cells from peripheral blood mononuclear cells (PBMCs) or purified NK cells. The CD2 and NKp46 antibodies provide key activating signals for NK cells. The Cloudz kit containing humanized antibodies is designed to streamline translation to clinical manufacturing processes.  

Design and Build Your Own Screening Cloudz (Coming Soon!)

Screening Cloudz are derivatized with streptavidin which offers you the flexibility to conjugate them with any biotinylated ligand such as our precisely labeled Avi-tag biotinylated proteins. With Screening Cloudz you can easily evaluate multiple ligands for the best cell activation or expansion in your particular assay. Once you have identified the optimum combination of ligands, we can manufacture a Cloudz kit at the scale you need. 

Ligands are typically chosen based on their ability to provide activating signals to the target cell. This includes antibodies to cell surface molecules and ligands that bind cell surface receptors. Inhibitory signals can be delivered by targeting inhibitory receptors or by using Cloudz derivatized with blocking antibodies or antagonistic ligands.

We offer an extensive portfolio of premium reagents including recombinant proteins, antibodies, peptides, and small molecules. Our Custom Protein Conjugation & Biotinylation services and Custom Chemistry Services can modify the molecules of your choice.

Book an appointment with a Cell and Gene Therapy Specialist to discuss the possibility of using Screening Cloudz in your application.

Not Seeing the Ligands You Need? Let Us Customize Your Cloudz.

Our Custom Services team will work with you to produce it. We have access to all Bio-Techne catalog products as well as hundreds of non-catalog reagents, and we can also custom design ligands for your optimum Cloudz kit. When you are ready to take the step to GMP-grade materials, we can deliver that too. Contact our Custom Team to learn more.

Bio-Techne believes in the power of partnerships. We have joined with Fresenius Kabi and Wilson Wolf to bring you ScaleReady™ for immune cell therapy manufacturing.

ScaleReady brings cell and gene therapies to life by streamlining manufacturing processes across cell culture, cell activation and expansion, gene editing, and cell processing.

Learn More at ScaleReady.com