Cloudz™ Cell Activation Kits

Cloudz immune cell therapy kits

Dissolvable Microspheres for Immune Cell Expansion in Cell Therapy

The robust ex vivo expansion of immune cells is a pivotal technique for the development and success of immune cell culture and immune cell therapies. R&D Systems is proud to provide a magnetic-free bead-based technology for the robust and reliable expansion of immune cells. Our pioneering Cloudz expansion technology provides a simple protocol for the expansion of several types of immune cells from a variety of cell sources, including peripheral blood mononuclear cells (PBMCs) and CD34+ T cell lines. The Cloudz expansion technology is:

Simple

Addition of release buffer instantly dissolves Cloudz microspheres for easy cell harvesting.

Fast, easy cell harvesting with addition of release buffer

Magnetic-Free

Magnetic-free separation avoids additional processing that can cause greater cell loss.

Avoid additional processing that causes greater cell loss

Optimized

Cloudz kits are ready to use with cytokines, growth factors and media from R&D Systems.

Ready to use with cytokines, growth factors and media from R&D Systems

 
Cloudz™ microspheres with CD3 and CD28 antibodies for T cell expansion

 

GMP Cloudz Human CD3/CD28 Cell Expansion Kit

T cells can be used as a powerful immunotherapy to fight against cancer and other diseases. The GMP Cloudz Human T Cell Activation Kit utilizes dissolvable CD3/CD28 microspheres that can easily be removed from expanded cell cultures. The GMP Cloudz Human T Cell Activation kit supports the expansion of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.

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Cloudz™ microspheres displaying CD2 and NKp46 antibodies for NK cell expansion

 

Cloudz Human NK Cell Expansion Kit

Natural killer cells are innate lymphoid cells with intrinsic cytoxic and cytokine producing capabilities that play an important role in infection, autoimmunity, and tumor immunosurveillance. The Cloudz Human NK Cell Expansion Kit provides a feeder-free expansion protocol that selectively expands NK cells from peripheral blood mononuclear cells (PBMCs).

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Cloudz™ microspheres displaying CD3 and CD28 antibodies for Treg expansion

 

Cloudz Human Regulatory T Cell (Treg) Expansion Kit

Regulatory T cells, also known as suppressor cells, are a subset of CD4+ T cells that maintain immune homeostasis by suppressing immune responses and controlling autoimmune recognition. The Cloudz Human Treg Expansion Kit provides robust expansion of FoxP3+ human Treg cells from CD4+ T cells.

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Expansion of Human T Cells using the Cloudz™ Human T Cell Activation Kit

 

GMP Cloudz Human CD3/CD28 Cell Expansion Kit

Expansion of Human T Cells using the Cloudz Human T Cell Activation Kit. Primary human CD3+ cells were activated with GMP Cloudz Human T Cell Activation Kit and cultured for 9 days in ExCellerate Human T Cell Expansion Media and 20 ng/mL GMP Recombinant Human IL-2. A. Cell counts were performed to determine fold expansion from the Day 0 seeding density (0.25 x 106 cells/mL). B. Percent Viability was determined on Days 5, 7, and 9.

 

Flow Cytometry Analysis of Expanded Human NK Cells

 

Cloudz Human NK Cell Expansion Kit

Flow Cytometry Analysis of Expanded Human NK Cells. Human PBMCs were expanded in vitro for 10 days using the protocol and reagents included in the Cloudz NK Cell Expansion Kit. At Day 10 of expansion, NK cells were collected, stained with NCAM-1/CD56 and Human CD3 epsilon Fluorescein-conjugated Antibody, and analyzed by flow cytometry. Compared to unexpanded PBMCs on Day 0 (A), PBMCs expanded with the Cloudz Human NK Cell Expansion Kit (B) showed an increased number of CD3+CD56+ NK cells.

 

Increased FoxP3-positive CD4+ T Cells Following Treg Cell Expansion

 

Cloudz Human Treg Expansion Kit

Increased FoxP3-positive CD4+ T Cells Following Treg Cell Expansion. Flow cytometry analysis of the Treg cell marker, FoxP3, in CD4+ T cells on Day 0 (A,C) and Day 9 (C, D) following expansion using the Cloudz Human Treg Expansion Kit. After nine days of expansion, cells were fixed, permeabilized, and stained with CD4-FITC, CD25-APC, and FoxP3-PE antibodies. Cells in panels (A) and (B) were stained for FoxP3 and CD4. Cells in (C) and (D) were stained for FoxP3 and CD25.