The Cloudz T Cell Activation Kit - CD3/CD28 contains non-magnetic, dissolvable microparticles that are manufactured under GMP controls and conjugated with the common T cell-activating antibodies, CD3 and CD28. This kit improves T cell expansion and simplifies cell recovery for ex vivo manufacturing, while also providing safety and efficacy advantages over current bead-based T cell activation methods.
- CD3/CD28 antibody-conjugated microparticles
- Release buffer dissolves particles in < 10 seconds
- Manufactured under GMP controls
- Ideal for CAR T or T cell therapeutic manufacturing
- Robust T cell activation
- Simplified recovery of cells
- Improved product purity over magnetic activation techniques
Cloudz CD3/CD28 Protocol
Advantages of Cloudz CD3/CD28 for Cell Therapy
Robust CD3+ T Cell Expansion
Cloudz CD3/CD28 induces 8-9 population doublings during a 9-day ex vivo culture of human CD3+ T cells or PBMCs.
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Favorable Performance Over Magnetic Bead and Soluble Antibody
With comparable expansion rates to existing bead and soluble antibody activating protocols, Cloudz CD3/CD28 offers additional manufacturing advantages, including single administration use and easier cell collection.
Independent analysis of Cloudz CD3/CD28 shows improved expansion over alternative expansion technologies. *Results disclosed with permission of external collaborator.