CometAssay 20-well Electrophoresisis Starter Kit

Catalog # Availability Size / Price Qty
4252-040-ESK
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CometAssay 20-well Electrophoresisis Starter Kit Summary

Designed as a standardized platform for running the Comet assay.

What is the CometAssay?

The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.

Why Use the CometAssay 20-well Electrophoresis Starter Kit?

CometAssay 20-well Electrophoresisis Starter Kit is designed to eliminate known causes of comet assay variability. It provides an exclusive 20-well CometSlide that is specially treated to promote adherence of low melting point agarose. This eliminates the time consuming and unreliable traditional method of preparing base layers of agarose. The use of 20-well CometSlide High Throughput shortens the overall assay time and allows for the rapid and reliable analysis of large numbers of samples.

Kit Contents

• CometAssay Electrophoresis System
• CometAssay Alkaline Control Cells
• CometAssay Lysis Solution
• CometAssay LMAgarose
• 200 mM EDTA, pH 10
• CometSlide  (20 Well, 2 Slides)

Kit Contents

  • CometAssay Electrophoresis System
  • CometAssay Alkaline Control Cells
  • CometAssay Lysis Solution
  • CometAssay LMAgarose
  • 200 mM EDTA, pH 10
  • CometSlide  (20 Well, 2 Slides)

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

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