Cotton Rat CD4 PE-conjugated Antibody

FAB6676P has been discontinued. View all CD4 products.
  
  • Species Reactivity
    Cotton Rat
  • Specificity
    Detects cotton rat CD4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD4 from human, mouse, rat, cat, or dog is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 695542
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant cotton rat CD4
    Lys26-Pro393
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD4 in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB6676P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD4
CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and the Th1, Th2, Th17, Tfh, and Treg subsets of mature T lymphocytes. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binding to MHC class II molecules on antigen presenting cells contributes to the formation of the immunological synapse. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.
  • Entrez Gene IDs:
    920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
  • Alternate Names:
    CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas

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