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Cultrex PathClear Reduced Growth Factor BME

3433-005-01 29 Citations Datasheet / COA / SDS
Newer Version Available: 3433-005-02
Basement Membrane Extract
Catalog # Availability Size / Price Qty
3433-005-01
Product Details
Citations (29)
FAQs
Reviews
Summary Product Datasheets Reconstitution Calculator Background Related Research Areas

Cultrex PathClear Reduced Growth Factor BME Summary

Endotoxin Level
<8.0 EU per 1 μg of the protein by the LAL method.
Activity
Functional Assay - Tube formation assay: BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Source

Murine Engelbreth-Holm-Swarm (EHS) tumor.

Product Datasheets

Product Datasheet
COA
SDS

3433-005-01

Formulation 12 - 18 mg/ml.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Product is stable for a minimum of 3 months from date of shipment when stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles.

Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.

Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
Reconstitution Calculator

Reconstitution Calculator

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Background: Basement Membrane Extracts

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells.  Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan.  BME can be used in a multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.

Alternate Names
Basement Membrane Extracts

Citations for Cultrex PathClear Reduced Growth Factor BME

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. MicroRNA-181a promotes angiogenesis in colorectal cancer by targeting SRCIN1 to promote the SRC/VEGF signaling pathway
    Authors: W Sun, X Wang, J Li, C You, P Lu, H Feng, Y Kong, H Zhang, Y Liu, R Jiao, X Chen, Y Ba
    Cell Death Dis, 2018;9(4):438.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization Controls Endochondral Ossification through Hyaluronan Metabolism
    Authors: M Shimoda, H Yoshida, S Mizuno, T Hirozane, K Horiuchi, Y Yoshino, H Hara, Y Kanai, S Inoue, M Ishijima, Y Okada
    Am. J. Pathol, 2017;0(0):.
  3. Bystander cells enhance NK cytotoxic efficiency by reducing search time
    Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
    Sci Rep, 2017;7(0):44357.
  4. Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways
    Authors: SA Ibrahim, R Gadalla, EA El-Ghonaim, O Samir, HT Mohamed, H Hassan, B Greve, M El-Shinawi, MM Mohamed, M Götte
    Mol. Cancer, 2017;16(1):57.
  5. Wnt signaling promotes breast cancer by blocking ITCH-mediated degradation of the YAP/TAZ transcriptional coactivator WBP2
    Cancer Res, 2016;0(0):.
  6. Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression
    Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):.
  7. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  8. miR-9 Acts as an OncomiR in Prostate Cancer through Multiple Pathways That Drive Tumour Progression and Metastasis
    PLoS ONE, 2016;11(7):e0159601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Influence of biological matrix and artificial electrospun scaffolds on proliferation, differentiation and trophic factor synthesis of rat embryonic stem cells.
    Authors: Alessandri M, Lizzo G, Gualandi C, Mangano C, Giuliani A, Focarete M, Calza L
    Matrix Biol, 2014;33(0):68-76.
  10. The M33 G protein-coupled receptor encoded by murine cytomegalovirus is dispensable for hematogenous dissemination but is required for growth within the salivary gland.
    Authors: Bittencourt F, Wu S, Bridges J, Miller W
    J Virol, 2014;88(20):11811-24.
  11. Therapeutic angiogenesis of mouse hind limb ischemia by novel peptide activating GRP78 receptor on endothelial cells.
    Authors: Hardy B, Battler A, Weiss C, Kudasi O, Raiter A
    Biochem. Pharmacol., 2007;75(4):891-9.
    Species: Human
    Sample Types: Whole Cells
  12. Silencing of hyaluronan synthase 2 suppresses the malignant phenotype of invasive breast cancer cells.
    Authors: Li Y, Li L, Brown TJ, Heldin P
    Int. J. Cancer, 2007;120(12):2557-67.
    Species: Human
    Sample Types: Whole Cells
  13. GPER mediates activation of HIF1alpha/VEGF signaling by estrogens.
    Authors: De Francesco E, Pellegrino M, Santolla M, Lappano R, Ricchio E, Abonante S, Maggiolini M
    Cancer Res, 0;74(15):4053-64.
  14. The hippo pathway effector YAP regulates motility, invasion, and castration-resistant growth of prostate cancer cells.
    Authors: Zhang L, Yang S, Chen X, Stauffer S, Yu F, Lele S, Fu K, Datta K, Palermo N, Chen Y, Dong J
    Mol Cell Biol, 0;35(8):1350-62.
  15. Preclinical Antitumor Efficacy of BAY 1129980-a Novel Auristatin-Based Anti-C4.4A (LYPD3) Antibody-Drug Conjugate for the Treatment of Non-Small Cell Lung Cancer.
    Authors: Willuda J, Linden L, Lerchen H, Kopitz C, Stelte-Ludwig B, Pena C, Lange C, Golfier S, Kneip C, Carrigan P, McLean K, Schuhmacher J, von Ahsen O, Muller J, Dittmer F, Beier R, El Sheikh S, Tebbe J, Leder G, Apeler H, Jautelat R, Ziegelbauer K, Kreft B
    Mol Cancer Ther, 0;16(5):893-904.
  16. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs.
    Authors: Caceres P, Mendez M, Ortiz P
    J Biol Chem, 0;289(34):23951-62.
  17. Function of carbonic anhydrase IX in glioblastoma multiforme.
    Authors: Proescholdt M, Merrill M, Stoerr E, Lohmeier A, Pohl F, Brawanski A
    Neuro Oncol, 0;14(11):1357-66.
  18. PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells.
    Authors: Kolev V, Wright Q, Vidal C, Ring J, Shapiro I, Ricono J, Weaver D, Padval M, Pachter J, Xu Q
    Cancer Res, 0;75(2):446-55.
  19. Metformin Reduces Prostate Tumor Growth, in a Diet-Dependent Manner, by Modulating Multiple Signaling Pathways.
    Authors: Sarmento-Cabral A, L-Lopez F, Gahete M, Castano J, Luque R
    Mol Cancer Res, 0;15(7):862-874.
  20. Suppression of ERbeta signaling via ERbeta knockout or antagonist protects against bladder cancer development.
    Authors: Hsu I, Chuang K, Slavin S, Da J, Lim W, Pang S, O'Brien J, Yeh S
    Carcinogenesis, 0;35(3):651-61.
  21. Targeted inactivation of beta1 integrin induces beta3 integrin switching, which drives breast cancer metastasis by TGF-beta.
    Authors: Parvani J, Galliher-Beckley A, Schiemann B, Schiemann W
    Mol Biol Cell, 0;24(21):3449-59.
  22. Effects of ulipristal acetate on human embryo attachment and endometrial cell gene expression in an in vitro co-culture system.
    Authors: Berger C, Boggavarapu N, Menezes J, Lalitkumar P, Gemzell-Danielsson K
    Hum Reprod, 0;30(4):800-11.
  23. Combination of an allosteric Akt Inhibitor MK-2206 with etoposide or rapamycin enhances the antitumor growth effect in neuroblastoma.
    Authors: Li Z, Yan S, Attayan N, Ramalingam S, Thiele C
    Clin Cancer Res, 0;18(13):3603-15.
  24. CLM94, a novel cyclic amide with anti-VEGFR-2 and antiangiogenic properties, is active against primary anaplastic thyroid cancer in vitro and in vivo.
    Authors: Antonelli A, Bocci G, La Motta C, Ferrari S, Fallahi P, Ruffilli I, Di Domenicantonio A, Fioravanti A, Sartini S, Minuto M, Piaggi S, Corti A, Ali G, Di Desidero T, Berti P, Fontanini G, Danesi R, Da Settimo F, Miccoli P
    J Clin Endocrinol Metab, 0;97(4):E528-36.
  25. Harvest of superficial layers of fat with a microcannula and isolation of adipose tissue-derived stromal and vascular cells.
    Authors: Trivisonno A, Di Rocco G, Cannistra C, Finocchi V, Torres Farr S, Monti M, Toietta G
    Aesthet Surg J, 0;34(4):601-13.
  26. Isogenic blood-brain barrier models based on patient-derived stem cells display inter-individual differences in cell maturation and functionality.
    Authors: Patel R, Page S, Al-Ahmad A
    J Neurochem, 0;142(1):74-88.
  27. Conditioned medium from human amniotic mesenchymal stromal cells limits infarct size and enhances angiogenesis.
    Authors: Danieli P, Malpasso G, Ciuffreda M, Cervio E, Calvillo L, Copes F, Pisano F, Mura M, Kleijn L, de Boer R, Viarengo G, Rosti V, Spinillo A, Roccio M, Gnecchi M
    Stem Cells Transl Med, 0;4(5):448-58.
  28. KLF6 Suppresses Metastasis of Clear Cell Renal Cell Carcinoma via Transcriptional Repression of E2F1.
    Authors: Gao Y, Li H, Ma X, Fan Y, Ni D, Zhang Y, Huang Q, Liu K, Li X, Wang L, Gu L, Yao Y, Ai Q, Du Q, Song E, Zhang X
    Cancer Res, 0;77(2):330-342.
  29. PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor cell invasion.
    Authors: Mense S, Barrows D, Hodakoski C, Steinbach N, Schoenfeld D, Su W, Hopkins B, Su T, Fine B, Hibshoosh H, Parsons R
    Sci Signal, 0;8(370):ra32.

FAQs

  1. How does Cultrex® BME promote cell differentiation?

    • All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic substrates, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

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