<8.0 EU per 1 μg of the protein by the LAL method.
Functional Assay - Tube formation assay: BME promotes formation
of capillary-like structures by human (HBMVEC; HUVEC) or mouse
(SVEC4-10) endothelial cells.
Gelling Assay - BME gels in less than 30 minutes at 37°C, and maintains the gelled form in culture medium for a minimum of 14 days at 37°C.
Murine Engelbreth-Holm-Swarm (EHS) tumor.
Formulation 12 - 18 mg/ml.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Product is stable for a minimum of 3 months from date of shipment when
stored at –20°C in a manual defrost freezer. For optimal stability,
store at –80°C. Avoid freeze-thaw cycles.
Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate.
Sterility Testing: PathClear®
- Negative by PCR test for mycoplasma; 17 bacterial and virus strains
typically included in mouse antibody production (MAP) testing, plus 13
additional murine infectious agents including LDEV, for a total of 31
organisms and viruses. No bacterial or fungal growth detected after
incubation at 37°C for 14 days following USP sterility testing
Background: Basement Membrane Extracts
Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support cells and cell layers, but they also play an essential role in tissue organization that affects cell adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor cells. Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The extract gels at 37°C to form a reconstituted basement membrane. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycan. BME can be used in a multiple applications, under a variety of cell culture conditions, for maintaining growth or promoting differentiation of primary endothelial, epithelial, smooth muscle and stem cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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