CXCR3, also known as GPR9, is a member of the CXC chemokine receptor subfamily. Similar to other chemokine receptors, it is a G protein-coupled receptor (GPCR). In humans, two alternatively spliced CXCR3 isoforms, CXCR3 and CXCR3B, have been identified and have different chemokine binding affinities. Human CXCR3 is 368 amino acids (aa) in length with a predicted molecular weight of 41 kDa. CXCR3B contains an additional N-terminal sequence and is 415 aa in length with a predicted molecular weight of 46 kDa. Mouse and rat CXCR3 share 86% aa sequence identity with human CXCR3. CXCR3 is expressed on leukocytes and is upregulated following T cell activation. It binds multiple CXC chemokines and is known to mediate leukocyte trafficking. CXCR3 has been implicated in neuroinflammatory and autoimmune diseases.
Rat CXCR3 ELISA Kit (Chemiluminescence)
Novus Biologicals | Catalog # NBP2-62863
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
18.75 pg/mL
Assay Range
31.25-2000 pg/mL
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Product Specifications
Assay Type
Sandwich-CLIA
Kit Type
ELISA Kit (Chemiluminescence)
Species
Rat
Specificity
This kit recognizes Rat CXCR3 in samples. No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 9.17%
Inter-Assay Precision (Precision between assays) CV% 9.17%
Recovery for Rat CXCR3 ELISA Kit (Chemiluminescence)
Recovery
87-112%
Linearity
Scientific Data Images for Rat CXCR3 ELISA Kit (Chemiluminescence)
ELISA: Rat CXCR3 ELISA Kit (Chemiluminescence) [NBP2-62863] -
ELISA: Rat CXCR3 ELISA Kit (Chemiluminescence) [NBP2-62863] - Standard Curve ReferenceKit Contents for Rat CXCR3 ELISA Kit (Chemiluminescence)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CXCR3
Alternate Names
CD183, CXCR3, GPR9
Gene Symbol
CXCR3
Additional CXCR3 Products
Product Documents for Rat CXCR3 ELISA Kit (Chemiluminescence)
Product Specific Notices for Rat CXCR3 ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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