Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2a Kappa Clone # rKRT8/4209

Format

Azide and BSA Free
View all Cytokeratin 8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human KRT8 protein (Uniprot: P05787)

Localization

Cytoplasm, Nucleoplasm, Nucleus, Nucleus matrix

Specificity

Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free [NBP3-49453] -

Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free.
Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free [NBP3-49453] -

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.

Applications for Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free and earn rewards!

Have you used Cytokeratin 8 Antibody (rKRT8/4209) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies