DYKDDDDK Epitope Tag HRP-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Specificity
    Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1042E
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    DYKDDDDK epitope tag
  • Formulation
    Supplied as a solution in PBS containing a preservative.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000-1:3000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DYKDDDDK (FLAG®) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-DYKDDDDK Epitope Tag HRP-conjugated Polyclonal Antibody (Catalog # HAM85291). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.

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