Cytokine Immunoassay Kits

ELISA Kit Formats for Cytokine Profiling

ELISAs for Chemokine Targets

ELISAs for Interleukin Targets

ELISAs for Other Cytokine Targets

Proteome Profiler^™ Antibody Arrays

Luminex^® Panels for Multiplex Assays

Cytokine profiling and quantification are essential for characterizing immune responses, detecting disease-associated biomarkers, and monitoring responses to therapy. To support these studies, R&D Systems offers a wide selection of enzyme-linked immunosorbent assays (ELISAs) for cytokine analysis.

R&D Systems ELISA Kits provide the highest levels of performance and consistency in analyte quantification. Built using our own in-house antibodies, proteins, and diluents, each kit component and complete kit are carefully tested throughout development and manufacturing. This allows us to ensure that every R&D Systems ELISA meets our rigorous standards for specificity, recovery, linearity, and precision, so you can have confidence in your results from one experiment to the next.

View All Cytokine ELISA Kits

Benefits of R&D Systems ELISAs

• Most Referenced ELISA Manufacturer
• Rigorous Validation Testing
• Long-term Consistency

• Exceptional Analyte Selection
• Extensive Quality Control Testing
• Bulk Packaging Available

Learn more about R&D Systems ELISA Quality Manufacturing and Validation Testing.

ELISA Kit Formats for Cytokine Profiling

We offer a broad range of single analyte immunoassay formats for cytokine quantification. These include our complete, ready-to-use Quantikine^™ ELISA Kits, our 90-minute QuicKit^™ ELISAs, and the more versatile DuoSet^™ ELISA Development Systems that provide all the components necessary for customers to develop their own working assays.

Quantikine™ ELISA Kits

• Extensively tested for long-term consistency and reproducibility
• Fully validated sample types
• Optimized reagents and diluents for accurate and sensitive sample values

Discover Quantikine Quality

Quantikine QuicKit^™ ELISA Kits

• Data generated in 90 minutes
• Quantikine quality with lot-to-lot consistency and reproducibility
• Simplified protocol – only one wash step

Get Results Faster

DuoSet^™ ELISA Development Systems

• Carefully selected and validated matched antibody pairs for optimal performance
• Large menu – over 1,000 targets with novel targets across multiple species
• Adaptable for use across multiple platforms and 384 well

Explore Development Reagents

Select Single Analyte ELISAs for Chemokine Targets

Select Single Analyte ELISAs for Interleukins/Interferons and their Soluble Receptors

Select Single Analyte ELISAs for Other Cytokines and Soluble Receptors

ELISpot Kits and ELISpot Development Modules for Detecting Cytokine-Secreting Cells

ELISpot Kits are highly sensitive, microplate-based assays for the detection of cytokine-secreting cells. Complete ELISpot Kits are ready-to-run and require no assay development or refinement. As an alternative to our complete kits, we also offer ELISpot Development Modules, which provide a flexible, do-it-yourself format for ELISpot development.

• Accurate quantitation – Small, crisp spots allow for simple and more quantitation and the largest dynamic range (number of detectable spots/well) on the market
• High sensitivity – ELISpot Assays can measure responses with frequencies below 1 in 100,000 cells – up to 20% more sensitive than our competition
• Assurance in the assay – We are the only company that provides recombinant protein positive controls

Learn more about ELISpot Assays.

Multiplex Assays for Cytokine Analysis

Proteome Profiler^™ Cytokine Antibody Arrays

Proteome Profiler Antibody Arrays are membrane-based arrays designed to simultaneous measure up to 119 proteins in a single sample. They provide a simple, economical, semi-quantitative multiplex assay for early stage discovery research. The arrays utilize chemiluminescence for detection, so membranes can be assessed for protein levels in the same manner as traditional Western blots.

Learn more about Proteome Profiler Antibody Arrays.

Luminex^® High Performance Cytokine Panels

Luminex panels are bead-based multiplex assays that enable you to multiplex up to 50 analytes, conserving on sample, time, and cost. These fully validated panels are our most accurate and precise bead-based multiplex assays for measuring target analytes in qualified complex sample types. They are our most accurate and precise bead-based multiplex assays for measuring target analytes in qualified complex sample types. All analytes in a given panel are optimized and tested together to ensure maximum performance and accuracy for the entire panel. Additionally, kits undergo similar validation testing as our single analyte Quantikine ELISA Kits and are correlated with our Quantikine ELISAs, when available.

Luminex High Performance Panels - Fixed

Fixed Luminex High Performance Panels provide a stocked and ready to ship option with shorter shipping times so you can begin your research more quickly.

Luminex High Performance Panels – Configurable

Configurable Luminex High Performance Panels allow users to choose from a selection of the analytes in the panel or the entire panel. They are supplied as either premixed or user-mixed panels as indicated on the kit specific product pages.

Learn more about Luminex Assays.

Featured Content

ELISA Tips and Tricks Poster

Learn tips on how to obtain consistent and reproducible data, ensure assay accuracy, and increase efficiency and speed.

ELISA Guide

Explore our comprehensive guide to running a successful ELISA, including best practices, controls and troubleshooting.

Periodic Table of Human Cytokine Families Poster

We love to make and measure cytokines and put them together in interesting ways. Check out our latest poster, it’s sure to brighten up your lab.

Handbooks and Guides

Four Fundamentals of Immunoassay Quality

ELISA Troubleshooting Guide

ELISA Sample Preparation and Collection Guide

Application Notes

Avoid False Positive Data: Improve Performance with R&D Systems^™ Quantikine ELISAs

Protocols

ELISA Protocols

Luminex Protocols

Posters

T Cell Subsets

Chemokine Superfamily

Periodic Table of Human Cytokine Families