FBXO25 Antibody (2F1) - Azide and BSA Free

Novus Biologicals | Catalog # H00026260-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F1

Format

Azide and BSA Free
View all FBXO25 Primary Antibodies »

Product Specifications

Immunogen

FBXO25 (AAH50393, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKDHFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQQQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEKQFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPDSCFTPVSPQHFIDLFKF

Reactivity Notes

Human. Other species not tested.

Specificity

FBXO25 - F-box protein 25

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for FBXO25 Antibody (2F1) - Azide and BSA Free

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - FBXO25 monoclonal antibody (M01), clone 2F1 Analysis of FBXO25 expression in COLO 320 HSR.
Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - FBXO25 monoclonal antibody (M01), clone 2F1 Western Blot analysis of FBXO25 expression in COLO 320 HSR ( Cat # L020V1 ).
Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01]

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - analysis of FBXO25 expression in transfected 293T cell line by FBXO25 monoclonal antibody (M01), clone 2F1. Lane 1: FBXO25 transfected lysate (43 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: FBXO25 Antibody (2F1) [H00026260-M01] - Detection limit for recombinant GST tagged FBXO25 is approximately 3ng/ml as a capture antibody.

Applications for FBXO25 Antibody (2F1) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FBXO25

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

F-box protein 25, F-box protein Fbx25, FBX25F-box only protein 25, MGC20256, MGC51975

Entrez Gene IDs

26260 (Human)

Gene Symbol

FBXO25

OMIM

609098 (Human)

UniProt

AAH50393 (Human)

Additional FBXO25 Products

Product Documents for FBXO25 Antibody (2F1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FBXO25 Antibody (2F1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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