Detects feline CXCL8/IL-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with over 75 tested chemokines of human, mouse, rat, porcine, or viral origin was observed.
Monoclonal Rat IgG2B Clone # 344609
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant feline CXCL8/IL‑8 Ala23-Ala101 Accession # Q9XSX5.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.08-0.32 µg/mL in the presence of 20 ng/mL Recombinant Feline CXCL8/IL‑8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Feline CXCL8/IL‑8 Antibody. Recombinant Feline CXCL8/IL‑8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CXCL8/IL‑8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB2277). The ND50 is typically 0.08-0.32 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.
Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271.
Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508.
Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43.