Recombinant Feline CXCL8/IL-8 Protein (Catalog # 2277-FL)
Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.075-0.375 µg/mL in the presence of 20 ng/mL Recombinant Feline CXCL8/IL‑8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Feline CXCL8/IL‑8 Antibody. Recombinant Feline CXCL8/IL‑8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CXCL8/IL‑8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB22772). The ND50 is typically 0.075‑0.375 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL8/Interleukin-8 is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. CXCL8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. CXCL8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents CXCL8 interaction with CXCR1.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.