GABA-A-R delta N-Terminus Affinity Purified PAb

PPS090 has been discontinued and is replaced by PPS090B.
    
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  • Species Reactivity
    Human, Mouse, Rat, Bovine
  • Specificity
    Human, mouse, rat, and bovine ~52 kDa GABAA R δ subunit, N-Terminus
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Fusion protein from the cytosolic loop of the rat GABAA R δ subunit, N-Terminus
  • Formulation
    100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunohistochemistry
    1:250 dilution
    (frozen sections; unpublished observations)
  • Immunoprecipitation
    10 μL/50 μg cell lysate
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of GABAA R δ N-Terminus by Western Blot. Western blot of mouse cerebellar lysates from wild type (Control) and δ knockout (KO) animals showing specific immunolabeling of the ~52 kDa δ subunit of the GABAA R. The labeling was absent from a lysate prepared from knockout animals.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R delta
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. Receptors in this group operate as GABA-gated Cl- channels. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Typical GABAA receptors have some combination of an alpha, beta, and gamma subunit. In select neurons, however, a δ subunit replaces the gamma -subunit. It would appear that δ subunits have a preference for various alpha -beta combinations. In cerebellar granule cells, the δ subunit contributes to a unique alpha 6 beta δ heteromer. δ subunit receptors may function to limit the spread of excitatory impulses to dendritically-complexed neurons. They also appear to be sensitive to steroid modulation. The rat δ subunit is a 50 kDa, 433 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 4 - 246). The rat and mouse N-terminal extracellular domains (ECD) (aa 17 - 248) are 99% aa identical; the mouse and human ECD are 95% aa identical.
  • References:
    1. Whiting, P.J. et al. (1999) Ann. N.Y. Acad. Sci. 868:645.
    2. Rudolph, U. et al. (2001) Trends Pharmacol. Sci. 22:188.
    3. Shivers, B.D. et al. (1989) Neuron 3:327.
    4. Sommer, B. et al. (1990) DNA Cell Biol. 9:561.
    5. Bianchi, M.T. and R.L. MacDonald (2003) J. Neurosci. 23:10934.
  • Long Name:
    gamma-Aminobutyric Acid Type A Receptor, delta Polypeptide
  • Entrez Gene IDs:
    2563 (Human); 14403 (Mouse); 29689 (Rat)
  • Alternate Names:
    EIG10; EJM7; GABA A R delta; GABA(A) receptor subunit delta; GABA-A receptor, delta polypeptide; GABAAR delta; GABAARd; GABRD; gamma-aminobutyric acid (GABA) A receptor, delta; gamma-aminobutyric acid receptor subunit delta; GEFSP5; MGC45284
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