GFP Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-37821

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Jellyfish

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GFP Primary Antibodies »

Product Specifications

Immunogen

This GFP antibody was developed against purified recombinant eGFP

Specificity

GFP antibody detects eGFP in samples. This product has been successfully used in Western Blot and ICC/IF applications. The immunogen is purified recombinant eGFP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GFP Antibody - Azide and BSA Free

Western Blot: GFP Antibody [NBP2-37821]

Western Blot: GFP Antibody [NBP2-37821]

Western Blot: eGFP Antibody [NBP2-37821] - WB analysis on HEK293 cells transfected with Green Fluorescent Protein (GFP) using eGFP antibody. 20ug whole cells lyate were loaded. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-37821]

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-37821]

Immunocytochemistry/Immunofluorescence: eGFP Antibody [NBP2-37821] - Analysis of eGFP using either natural fluorescence (green) or an eGFP antibody (red) in U2OS cells transfected with an EGFR-eGFP fusion protein.
Western Blot: GFP Antibody [NBP2-37821]

Western Blot: GFP Antibody [NBP2-37821]

Western Blot: eGFP Antibody [NBP2-37821] - 12% SDS-PAGE of HEK-293 cells transfected with pCMV-Myc-eGFP (0.25ug DNA lanes 2 and 4; 0.50ug DNA lanes 3 and 5) Coomassie stain (lanes 2 and 3) and western blot (lanes 4 and 5) with anti-eGFP antibody and goat anti-rabbit HRP secondary

Applications for GFP Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP2-37821 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv, anti-gfp (green fluorescent protein) pab, clone 9F9.F9, GFP immunofluorescence, GFP immunoprecipitation, GFP monoclonal, GFP mouse, GFP staining, GFP western blot

Additional GFP Products

Product Documents for GFP Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GFP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GFP Antibody - Azide and BSA Free

Customer Reviews for GFP Antibody - Azide and BSA Free (1)

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Customer Images

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  • eGFP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Transfected HEK 293 cells
    Species: Non-species related
    Verified Customer | Posted 02/02/2017
    HEK293 cells were transfected with Green Fluorescent Protein (GFP), 20ug whole cells lyate were loaded. This is a clean antibody with almost no background at all.
    GFP Antibody - Azide and BSA Free NBP2-37821

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Protocols

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FAQs for GFP Antibody - Azide and BSA Free

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  • Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?

    A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.

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