Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H1

Format

BSA Free
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Product Specifications

Immunogen

The immunogen for this antibody was CD116.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GM-CSFR alpha Antibody (4H1) - BSA Free

Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Analysis using the PE conjugate of NBP1-43361. Analysis of human peripheral blood leukocytes using GM-CSF R alpha PE conjugate. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.
Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Surface staining of normal human peripheral blood cells with Anti-Human CD116 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.
Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSFR alpha Antibody (4H1) [NBP1-43361]

Flow Cytometry: GM-CSF R alpha Antibody (4H1) [NBP1-43361] - Human peripheral blood monocytes stained with 4H1 FITC.

Applications for GM-CSFR alpha Antibody (4H1) - BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The 4H1 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GM-CSF R alpha

The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells.

Long Name

Granulocyte Macrophage Growth Factor Receptor alpha

Alternate Names

CD116, CSF2R, CSF2RA, CSF2RY, GM-CSFRa, GMCSFR alpha

Gene Symbol

CSF2RA

Additional GM-CSF R alpha Products

Product Documents for GM-CSFR alpha Antibody (4H1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GM-CSFR alpha Antibody (4H1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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