Key Product Details

Species Reactivity

Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgY Secondary Antibodies »

Product Specifications

Immunogen

Chicken IgY

Specificity

Detects chicken IgY heavy and light chains.  In direct ELISA, less than 1% cross-reactivity with human IgG, rabbit IgG, rat IgG, donkey IgG and mouse IgG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat Anti-Chicken IgY Antibody

Application
Recommended Usage

Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody

Reviewed Applications

Read 2 reviews rated 4.5 using AF010 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgY

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin Y

Additional IgY Products

Product Documents for Goat Anti-Chicken IgY Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Goat Anti-Chicken IgY Antibody

For research use only

Citations for Goat Anti-Chicken IgY Antibody

Customer Reviews for Goat Anti-Chicken IgY Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Goat Anti-Chicken IgY Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Chicken
    Verified Customer | Posted 05/01/2018
  • Goat Anti-Chicken IgY Antibody
    Name: Jagadeesh Janjanam
    Application: Western Blot
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 06/13/2017
    Plasma sample 10 µg / well Antigen: alpha -Fetoprotein (AFP) Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369 Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010 Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020) Molecular weight of the antigen: 68 kDa Percentage of SDS-PAGE gel: 12.5 % Run time: 3.5 hours Wet transfer @ 100 V for 1 hr
    Goat Anti-Chicken IgY Antibody AF010

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FAQs

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