Goat Anti-Chicken IgY Antibody

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Goat Anti-Chicken IgY Antibody Summary

Species Reactivity
Detects chicken IgY heavy and light chains.  In direct ELISA, less than 1% cross-reactivity with human IgG, rabbit IgG, rat IgG, donkey IgG and mouse IgG is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Chicken IgY
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.


Recommended Concentration
Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgY

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin Y
Alternate Names

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Citation for Goat Anti-Chicken IgY Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008;181(4):2772-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Secondary Antibodies

Reviews for Goat Anti-Chicken IgY Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Goat Anti-Chicken IgY Antibody
By Danlee Enzler on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Chicken

Goat Anti-Chicken IgY Antibody
By Jagadeesh Janjanam on 06/13/2017
Application: Western Blot Sample Tested: Plasma Species: Human

Plasma sample 10 µg / well

Antigen: α-Fetoprotein (AFP)

Primary antibody: Anti-Human AFP antibody (Polyclonal Chicken IgY) from R&D systems; Cat No. AF1369

Secondary antibody_1: Goat anti-chicken IgY-unconjugated (Dilution: 1:5000) from R&D systems; Cat No. AF010

Secondary antibody_2: Donkey anti-goat IgG-HRP from Santa Cruz; Cat no. sc-2020)

Molecular weight of the antigen: 68 kDa

Percentage of SDS-PAGE gel: 12.5 %

Run time: 3.5 hours

Wet transfer @ 100 V for 1 hr