Goat Anti-Rabbit IgG Biotinylated Antibody

  (4 citations)
(1 Review)
  
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Rabbit
  • Specificity
    Detects rabbit IgG heavy and light chains. In direct ELISAs,  less than 5% cross-reactivity with human IgG, mouse IgG and chicken IgY is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rabbit IgG
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:5000 dilution
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. The expression of SIRT1 regulates the metastaticplasticity of chondrosarcoma cells by inducing epithelial-mesenchymal transition
    Authors: H Feng, J Wang, J Xu, C Xie, F Gao, Z Li
    Sci Rep, 2017;7(0):41203.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study.
    Authors: Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P
    BMC Nephrol, 2013;14(0):80.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  3. Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines.
    Authors: Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder SC, Li DQ
    Invest. Ophthalmol. Vis. Sci., 2009;50(6):2702-9.
    Species: Human
    Sample Type: Antibody
    Application: IHC Frozen
  4. A novel role of the lumican core protein in bacterial lipopolysaccharide-induced innate immune response.
    Authors: Wu F, Vij N, Roberts L, Lopez-Briones S, Joyce S, Chakravarti S
    J. Biol. Chem., 2007;282(36):26409-17.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Development

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Multi-Species.
We have 1 review tested in 1 application: Immunoblot.

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Very Good
 Immunoblot Multi-Species Anonymous 02/22/2016
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Summary

Application Immunoblot
Sample Tested Cell Culture Samples
Species Multi-Species

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