Intracellular Staining by Flow Cytometry
|Detection of Hemagglutinin/HA in HEK293 Human Cell Line Transfected with Hemagglutinin/HA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) Hemagglutinin/HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-Hemagglutinin/HA Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC6875R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.|
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