|Detection of Hemagglutinin/HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Hemagglutinin/HA Biotinylated Monoclonal Antibody (Catalog # BAM0601) followed by Streptavidin-HRP (Catalog # DY998). Specific bands were detected for Hemagglutinin/HA-tagged proteins at approximately 70 and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided