* Provided that the recommended microplates, buffers, diluents, substrates and solutions
are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human 14-3-3 zeta. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: 14-3-3 zeta
14-3-3 proteins constitute a highly conserved family of homo- and heterodimeric proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs containing phosphorylated serine or threonine residues. There are at least seven distinct 14-3-3 genes in vertebrates: alpha/beta, epsilon, eta, gamma, theta, sigma, and zeta/delta.
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