Human 17 beta-HSD1/HSD17B1 Antibody

Catalog # Availability Size / Price Qty
MAB7178
MAB7178-SP
Detection of Human 17  beta ‑HSD1/HSD17B1 by Western Blot.
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Human 17 beta-HSD1/HSD17B1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human 17 beta ‑HSD1/HSD17B1 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 860020
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human 17 beta ‑HSD1/HSD17B1
Ala2-Gln328
Accession # P14061
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human 17  beta ‑HSD1/HSD17B1 by Western Blot. View Larger

Detection of Human 17 beta ‑HSD1/
HSD17B1 by Western Blot.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human 17  beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human 17  beta ‑HSD1/HSD17B1 by Simple WesternTM. View Larger

Detection of Human 17 beta ‑HSD1/HSD17B1 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human 17 beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Long Name
17 beta Hydroxysteroid Dehydrogenase 1
Entrez Gene IDs
3292 (Human); 15485 (Mouse); 25322 (Rat)
Alternate Names
17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human 17 beta -HSD1/HSD17B1 Antibody
By Anonymous on 03/27/2017
Application: WB Sample Tested: Endometrial epithelial Species: Human