17 beta ‑HSD14/HSD17B14 in Human Brain. 17 beta ‑HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta ‑HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 17 beta-HSD14/HSD17B14
(hydroxysteroid (17-beta) dehydrogenase type14), also called 17bHSD14 or DHRS10, is a
cytoplasmic 17beta-hydroxysteroid dehydrogenase that is a member of the SDR
(short-chain dehydrogenase/reductase) family. It is a 270 amino acid,
approximately 30 kDa protein with mRNA highly expressed in brain, placenta,
liver and kidney. It converts estradiol to estrone, thus inactivating it, and
is thought to regulate activity of estrogens and possibly
dihydroepiandrosterone (DHEA) in the placenta and central nervous system. Human
HSD-17b14 shares 80.5% amino acid sequence identity with mouse and rat
17 beta Hydroxysteroid Dehydrogenase 14
Entrez Gene IDs:
51171 (Human); 66065 (Mouse); 691018 (Rat)
17 betaHSD14; dehydrogenase/reductase (SDR family) member 10; Dehydrogenase/reductase SDR family member 10; DHRS10; DHRS1017-beta-hydroxysteroid dehydrogenase 14; EC 1.1.1.-; HSD17B14; hydroxysteroid (17-beta) dehydrogenase 14,17-beta-hydroxysteroid dehydrogenase DHRS10; retinal short-chain dehydrogenase/reductase 3; Retinal short-chain dehydrogenase/reductase retSDR3; retSDR3; RETSDR3,17-beta-HSD 14; SDR3; SDR47C1; short chain dehydrogenase/reductase family 47C, member 1
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