Human ADAMTS13 Quantikine ELISA Kit

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DADT130
Control Products Available
Human ADAMTS13 Standard Curve
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Product Details
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Citations (5)
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Human ADAMTS13 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
0.26 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human ADAMTS13. This kit also recognizes a truncated version of recombinant human ADAMTS13 (amino acids Gln34-Trp688; Accession # NP_620594).
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human ADAMTS13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ADAMTS13 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human ADAMTS13 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ADAMTS13 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ADAMTS13.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 4.78 13.8 29.7 4.51 14.2 30.2
Standard Deviation 0.177 0.364 0.685 0.256 0.722 1.08
CV% 3.7 2.6 2.3 5.7 5.1 3.6

Recovery

The recovery of ADAMTS13 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 100 91-111
Citrate Plasma (n=4) 97 87-114
EDTA Plasma (n=4) 97 89-109
Heparin Plasma (n=4) 97 85-115
Serum (n=4) 97 85-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAMTS13 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ADAMTS13 ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAMTS13

Members of the ADAMTS family contain pro, catalytic, disintegrin-like, cysteine-rich, spacer and a variable number of TS type 1 motifs. In contrast to membrane-anchored ADAMs, ADAMTSs are secreted and are all active proteases. They are essential for aggrecan turnover and procollagen processing, regulation of which is important in many physiological and pathological processes such as angiogenesis and arthritis.

Long Name:
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13
Entrez Gene IDs:
11093 (Human); 279028 (Mouse); 362091 (Rat)
Alternate Names:
A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human ADAMTS13 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Serum ADAMTS-13 Levels as an Indicator of Portal Vein Thrombosis
    Authors: T Miku?a, J Koz?owska, W Sta?czak, M Sapu?a, A Ró?yk, A Wierci?ska
    Gastroenterol Res Pract, 2018;2018(0):3287491.
    Species: Human
    Sample Types: Serum
  2. mRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice
    Authors: S Liu-Chen, B Connolly, L Cheng, RR Subramania, Z Han
    Sci Rep, 2018;8(1):7859.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Clinical and prognostic significance of serum levels of von Willebrand factor and ADAMTS-13 antigens in AL amyloidosis
    Blood, 2016;0(0):.
    Species: Human
    Sample Types: Serum
  4. Reduced ADAMTS13 activity is associated with an ADAMTS13 SNP, fever and microparticles in a malaria-like model.
    Authors: Kraisin S, Palasuwan A, Popruk S, Nantakomol D
    Malar J, 2014;13(0):3.
    Species: Human
    Sample Types: Plasma
  5. Effect of ADAMTS-13 on cerebrovascular microthrombosis and neuronal injury after experimental subarachnoid hemorrhage.
    Authors: Muroi C, Fujioka M, Mishima K, Irie K, Fujimura Y, Nakano T, Fandino J, Keller E, Iwasaki K, Fujiwara M
    J Thromb Haemost, 2014;12(4):505-14.
    Species: Mouse
    Sample Types: Whole Blood

FAQs

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Human ADAMTS13 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum