Human alpha 2-Macroglobulin Antibody

Catalog # Availability Size / Price Qty
AF1938
AF1938-SP
Human  alpha 2-Macroglobulin Antibody in Western Blot
3 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (4)

Human alpha 2-Macroglobulin Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human alpha 2-Macroglobulin-like 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human plasma-derived alpha 2-Macroglobulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Human alpha 2-Macroglobulin (Catalog # 1938-PI), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human  alpha 2‑Macroglobulin by Western Blot. View Larger

Detection of Human alpha 2‑Macroglobulin by Western Blot. Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human
alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).
A specific band was detected for
alpha 2‑Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry alpha 2‑Macroglobulin  in human PBMCs. View Larger

alpha 2‑Macroglobulin in human PBMCs.
alpha 2‑Macroglobulin was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human
alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection  of Human  alpha 2‑Macroglobulin by Simple  WesternTM. View Larger

Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 1 µg/mL of Goat Anti-Human alpha 2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 2-Macroglobulin

Human alpha 2-macroglobulin (h alpha 2M) is a serum glycoprotein that has sequence similarity to other members of the alpha 2M family including complement components C3, C4 and C5 (1). alpha 2M is synthesized as a polypeptide of 1474 amino acids with a signal peptide (23 residues) (2). The mature protein is a tetramer (720 kDa) of 4 identical subunits (180 kDa), which form two disulfide bond-linked dimers. As a general and irreversible protease inhibitor implicated in many processes, alpha 2M is able to inhibit all four classes of proteases by a unique trapping mechanism. The bait region of h alpha 2M (residues 690‑728) contains specific cleavage sites for different proteases. The cleavage of the bait region by a protease induces a conformation change in alpha 2M, which then traps and forms a covalent bond with the protease. The trapped protease remains active against small peptide substrates but loses its ability to interact with large protein substrates or inhibitors.

References
  1. Sottrup-Jensen, L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:9.
  2. Kan, C.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:2282.
Entrez Gene IDs
2 (Human)
Alternate Names
A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7

Product Datasheets

Citations for Human alpha 2-Macroglobulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
    Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
    EMBO Mol Med, 2014;6(1):27-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter.
    Authors: Kim JC, Kim JY, Yeom SR, Jeong BY, Hwang HZ, Park KJ, Lee SW
    Proteomics, 2008;8(17):3632-44.
    Species: Rat
    Sample Types: Serum
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human alpha 2-Macroglobulin Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human alpha 2-Macroglobulin Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human alpha 2-Macroglobulin Antibody
By Anonymous on 12/20/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human alpha 2-Macroglobulin Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human,Mouse

Human alpha 2-Macroglobulin Antibody
By Anonymous on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human