Human AMICA/JAML Fluorescein-conjugated Antibody

FAB34491F has been discontinued and is replaced by FAB34491G.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human AMICA/JAML in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse AMICA is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 401901
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human AMICA/JAML
    Leu20-Leu275
    Accession # Q86YT9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of AMICA/JAML in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human AMICA/JAML Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB34491F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: AMICA/JAML

AMICA (Adhesion Molecule, Interacting with CXADR Antigen 1), also known as JAML, is a 65 kDa, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells (1). The human AMICA cDNA encodes a 384 amino acid (aa) precursor that includes a 19 aa signal sequence, a 256 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain (2). Alternative splicing may generate isoforms with N- and C-terminal deletions. In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, human AMICA shares 58% and 63% aa sequence identity with mouse and rat AMICA, respectively. It shares 18%‑20% aa sequence identity with the ECDs of human JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is upregulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates the adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with the coxsackie virus and adenovirus receptor (CXADR) in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

  • References:
    1. Mandell, K.J. and C.A. Parkos (2005) Adv. Drug Deliv. Rev. 57:857.
    2. Moog-Lutz, C. et al. (2003) Blood 102:3371.
    3. Zen, K. et al. (2005) Mol. Biol. Cell 16:2694.
  • Long Name:
    Adhesion Molecule, Interacts With CXADR Antigen 1
  • Entrez Gene IDs:
    120425 (Human); 270152 (Mouse)
  • Alternate Names:
    adhesion molecule, interacts with CXADR antigen 1; AMICA; AMICA1; CREA7-1; Gm638; JAML
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