Human Angiopoietin-1 DuoSet ELISA

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Human Angiopoietin-1 ELISA Standard Curve
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Product Details
Citations (30)
Supplemental Products
Reviews (1)

Human Angiopoietin-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human Angiopoietin-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-1

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
284 (Human); 11600 (Mouse)
Alternate Names:
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

Citations for Human Angiopoietin-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Depolymerization of fucoidan with endo-fucoidanase changes bioactivity in processes relevant for bone regeneration
    Authors: J Ohmes, MD Mikkelsen, TT Nguyen, VHN Tran, S Meier, MS Nielsen, M Ding, A Seekamp, AS Meyer, S Fuchs
    Carbohydrate polymers, 2022;286(0):119286.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy
    Authors: V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain
    EBioMedicine, 2021;73(0):103683.
    Species: Mouse
    Sample Types: Plasma
  3. ICU Admission Levels of Endothelial Biomarkers as Predictors of Mortality in Critically Ill COVID-19 Patients
    Authors: AG Vassiliou, C Keskinidou, E Jahaj, P Gallos, I Dimopoulou, A Kotanidou, SE Orfanos
    Cells, 2021;10(1):.
    Species: Human
    Sample Types: Plasma
  4. Angiopoietin 1 release from human neutrophils is independent from neutrophil extracellular traps (NETs)
    Authors: E Charles, BL Dumont, S Bonneau, PE Neagoe, L Villeneuve, A Räkel, M White, MG Sirois
    BMC immunology, 2021;22(1):51.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Analysis of Inflammatory Mediator Profiles in Sepsis Patients Reveals That Extracellular Histones Are Strongly Elevated in Nonsurvivors
    Authors: T Eichhorn, I Linsberger, L Lauková, C Tripiscian, B Fendl, R Weiss, F König, G Valicek, G Miestinger, C Hörmann, V Weber
    Mediators of Inflammation, 2021;2021(0):8395048.
    Species: Human
    Sample Types: Plasma
  6. Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
    Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
    Scientific Reports, 2021;11(1):13100.
    Species: Human
    Sample Types: Serum
  7. Dysregulation of angiopoietin-Tie-2 axis in ugandan children hospitalized with pneumonia
    Authors: R Zhang, U Rai, NBM Ibrahim, Y Amazouz, J Soo, AL Conroy, S Namasopo, RO Opoka, R Bhargava, MT Hawkes
    Cytokine, 2020;133(0):155175.
    Species: Human
    Sample Types: Plasma
  8. Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
    Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
    Mar Drugs, 2020;18(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Association of Angiopoietin Dysregulation in Placental Malaria with Adverse Birth Outcomes
    Authors: PP Singh, S Bhandari, RK Sharma, N Singh, PK Bharti
    Dis. Markers, 2020;2020(0):6163487.
    Species: Human
    Sample Types: Plasma
  10. Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway
    Authors: KD Tang, J Liu, PJ Russell, JA Clements, MT Ling
    Int J Mol Sci, 2019;20(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Progestin-induced heart and neural crest derivatives-expressed transcript 2 inhibits angiopoietin 2 via fibroblast growth factor 9 in human endometrial stromal cells
    Authors: H Murata, T Tsuzuki, T Kido, M Kakita-Kob, N Kida, Y Hisamatsu, H Okada
    Reprod Biol, 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Hepatic blood flow by perfusion computed tomography as an imaging biomarker for patients with gastric cancer
    Authors: K Shuto, M Mori, C Kosugi, K Narushima, S Nakabayash, T Fujisiro, A Sato, K Hayano, H Shimizu, K Koda
    Oncol Lett, 2019;17(3):3267-3276.
    Species: Human
    Sample Types: Serum
  13. Tie2 protects the vasculature against thrombus formation in systemic inflammation
    Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  14. Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
    Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
    BMC Pediatr, 2018;18(1):118.
    Species: Human
    Sample Types: Plasma
  15. The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells.
    Authors: Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G
    Cytokine, 2015;71(2):154-60.
    Species: Human
    Sample Types: Serum
  16. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  17. Malaria in Pregnancy Interacts with and Alters the Angiogenic Profiles of the Placenta.
    Authors: Ataide R, Murillo O, Dombrowski J, Souza R, Lima F, Lima G, Hristov A, Valle S, Di Santi S, Epiphanio S, Marinho C
    PLoS Negl Trop Dis, 2015;9(6):e0003824.
    Species: Human
    Sample Types: Plasma
  18. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
  20. Increased endothelial inflammation, sTie-2 and arginase activity in umbilical cords obtained from gestational diabetic mothers.
    Authors: Giri H, Chandel S, Dwarakanath L, Sreekumar S, Dixit M
    PLoS ONE, 2013;8(12):e84546.
    Species: Human
    Sample Types: Serum
  21. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  22. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010;31(6):545-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
    Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
    PLoS ONE, 2010;5(12):e15291.
    Species: Human
    Sample Types: Plasma
  24. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Role of vitamin D receptor in the antiproliferative effects of calcitriol in tumor-derived endothelial cells and tumor angiogenesis in vivo.
    Authors: Chung I, Han G, Seshadri M, Gillard BM, Yu WD, Foster BA, Trump DL, Johnson CS
    Cancer Res., 2009;69(3):967-75.
    Species: Mouse
    Sample Types: Cell Lysates
  26. Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
    Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
    Pathol. Oncol. Res., 2009;15(2):193-201.
    Species: Human
    Sample Types: Serum
  27. Angiopoietin-1/angiopoietin-2 ratio for prediction of preeclampsia.
    Authors: Bolin M, Wiberg-Itzel E, Wikstrom AK, Goop M, Larsson A, Olovsson M, Akerud H
    Am. J. Hypertens., 2009;22(8):891-5.
    Species: Human
    Sample Types: Plasma
  28. Intradermal delivery of plasmid VEGF(165) by electroporation promotes wound healing.
    Authors: Ferraro B, Cruz YL, Coppola D, Heller R
    Mol. Ther., 2009;17(4):651-7.
    Species: Rat
    Sample Types: Cell Culture Supernates
  29. Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
    Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
    Br. J. Cancer, 2009;101(4):605-14.
    Species: Human
    Sample Types: Serum
  30. Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients.
    Authors: van der Heijden M, van Nieuw Amerongen GP, Koolwijk P, van Hinsbergh VW, Groeneveld AB
    Thorax, 2008;63(10):903-9.
    Species: Human
    Sample Types: Plasma


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Human Angiopoietin-1 DuoSet ELISA
By Anonymous on 10/07/2019
Sample Tested: Recombinant protein