Detection of Human APP+1 by Western Blot.
Western blot shows samples of Recombinant Human APP+1 Western Blot Standard (Catalog # WBC012) (10 ng). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human APP+1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF850) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for APP was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, ‑751, and ‑733 contain a Kunitz protease inhibitor (KPI) domain (residue 291‑342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta - and gamma -secretase cleave at the beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta ‑amyloid peptide (A beta 40 and A beta 42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha -secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).
Haass, C. (2004) EMBO J. 23:483.
Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.
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