Human APRIL/TNFSF13 APC-conjugated Antibody

Name: Human APRIL/TNFSF13 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC884A
Price: 411 USD

Catalog #: IC884A Datasheet / COA / SDS

Discontinued Product

IC884A has been discontinued.

View all APRIL/TNFSF13 products.

Detection of APRIL/TNFSF13 in Th2-stimulated PBMCs by Flow Cytometry.

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All APRIL/TNFSF13 Antibodies

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Human APRIL/TNFSF13 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APRIL/TNFSF13

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of APRIL/TNFSF13 antibody in Th2-stimulated PBMCs antibody by Flow Cytometry.

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Detection of APRIL/TNFSF13 in Th2-stimulated PBMCs by Flow Cytometry. Th2-stimulated peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human APRIL/TNFSF13 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC884A) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC108A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: APRIL/TNFSF13

APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5-7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7-9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with Heparan Sulfate Proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References

Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
Kelly, K. et al. (2000) Cancer Res. 60:1021.
Roth, W. et al. (2001) Cell Death Differ. 8:403.
Yu, G. et al. (2000) Nat. Immunol. 1:252.
He, B. et al. (2004) J. Immunol. 172:3268.
Schwaller, J. et al. (2007) Blood 109:331.
Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
Day, E.S. et al. (2005) Biochemistry 44:1919.
Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
Roschke, V. et al. (2002) J. Immunol. 169:4314.
Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Long Name

A Proliferation-inducing Ligand

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat); 102126587 (Cynomolgus Monkey)

Alternate Names

A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2