Human BDNF PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human BDNF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NT-3, rhNT-4, or rh beta -NGF is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 35909
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
    Accession # P23560
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of BDNF in U‑87 MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human BDNF PerCP‑conjugated Monoclonal Antibody (Catalog # IC2481C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

  • References:
    1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
    2. Snider, W.D. (1994) Cell 77:627.
    3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
  • Long Name:
    Brain-derived Neurotrophic Factor
  • Entrez Gene IDs:
    627 (Human); 12064 (Mouse); 24225 (Rat)
  • Alternate Names:
    Abrineurin; ANON2; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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