Human BMI-1 APC-conjugated Antibody

IC33341A has been discontinued and is replaced by IC33341R.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects human BMI-1 in direct ELISAs  and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 384515
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BMI-1
    Accession # P35226
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of BMI‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human BMI‑1 APC‑conjugated Monoclonal Antibody (Catalog # IC33341A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BMI-1

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa proto-oncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain within amino acids (aa) 17-56, a nuclear location sequence (NLS) at aa 81-95 and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.

  • Long Name:
    B Lymphoma Mo-MLV Insertion Region 1
  • Entrez Gene IDs:
    648 (Human); 12151 (Mouse)
  • Alternate Names:
    B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4
Related Research Areas


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