Human BMP-7 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DBP700
PDBP700
SBP700
Control Products Available
Human BMP-7 ELISA Cell Culture Supernate/Urine Standard Curve
3 Images
Product Details
Procedure
Citations (8)
FAQs
Supplemental Products
Reviews (3)

Human BMP-7 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Bone Extracts (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity
7.83 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Bone Extracts, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human BMP-7
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BMP-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-7 in bone tissue extracts, cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human BMP-7 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BMP-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Bone Extracts, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 365 783 1194 336 718 1110
Standard Deviation 22.1 35.9 80.7 31.9 64.9 86.4
CV% 6.1 4.6 6.8 9.5 9 7.8

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 370 814 1241 356 752 1148
Standard Deviation 20.6 26.8 40.9 27.5 54.5 81.5
CV% 5.6 3.3 3.3 7.7 7.2 7.1

Recovery

The recovery of BMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Bone Extraction Solution (n=2) 96 88-102
Cell Culture Media (n=4) 98 90-114
EDTA Plasma (n=4) 100 93-105
Heparin Plasma (n=4) 106 96-115
Serum (n=4) 100 90-105
Urine (n=4) 97 90-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BMP-7 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human BMP-7 ELISA Linearity

Data Examples

Human BMP-7 ELISA Cell Culture Supernate/Urine Standard Curve

Human BMP-7 ELISA Serum/Plasma/Bone Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-7

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name:
Bone Morphogenetic Protein 7
Entrez Gene IDs:
655 (Human); 12162 (Mouse); 85272 (Rat)
Alternate Names:
BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human BMP-7 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Serum Markers of Epithelial Mesenchymal Transition as Predictors of HCV-induced Liver Fibrosis, Cirrhosis and Hepatocellular Carcinoma.
    Authors: Zoheiry M, Hasan S, El-Ahwany E, Nagy F, Taleb H, Nosseir M, Magdy M, Meshaal S, El-Talkawy M, Raafat I
    Electron Physician, 2015;7(8):1626-37.
    Species: Human
    Sample Types: Serum
  2. Circulating bone morphogenetic protein-7 and transforming growth factor-beta1 are better predictors of renal end points in patients with type 2 diabetes mellitus.
    Authors: Wong M, Perkovic V, Woodward M, Chalmers J, Li Q, Hillis G, Yaghobian Azari D, Jun M, Poulter N, Hamet P, Williams B, Neal B, Mancia G, Cooper M, Pollock C
    Kidney Int, 2013;83(2):278-84.
    Species: Human
    Sample Types: Plasma
  3. Bone morphogenetic proteins and the polycystic ovary syndrome.
    Authors: van Houten E, Laven J, Louwers Y, McLuskey A, Themmen A, Visser J
    J Ovarian Res, 2013;6(1):32.
    Species: Human
    Sample Types: Serum
  4. Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis.
    Authors: Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL, Chou CT
    J. Rheumatol., 2010;37(10):2126-32.
    Species: Human
    Sample Types: Serum
  5. Bone morphogenetic protein 7 inhibits tumor growth of human uveal melanoma in vivo.
    Authors: Notting I, Buijs J, Mintardjo R, van der Horst G, Vukicevic S, Lowik C, Schalij-Delfos N, Keunen J, Van Der Pluijm G
    Invest. Ophthalmol. Vis. Sci., 2007;48(11):4882-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Combination of scaffold and adenovirus vectors expressing bone morphogenetic protein-7 for alveolar bone regeneration at dental implant defects.
    Authors: Zhang Y, Song J, Shi B, Wang Y, Chen X, Huang C, Yang X, Xu D, Cheng X, Chen X
    Biomaterials, 2007;28(31):4635-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Bone morphogenetic protein 7 is elevated in patients with chronic liver disease and exerts fibrogenic effects on human hepatic stellate cells.
    Authors: Tacke F, Gabele E, Bataille F, Schwabe RF, Hellerbrand C, Klebl F, Straub RH, Luedde T, Manns MP, Trautwein C, Brenner DA, Scholmerich J, Schnabl B
    Dig. Dis. Sci., 2007;52(12):3404-15.
    Species: Human
    Sample Types: Plasma
  8. Bone morphogenetic protein 7 in the development and treatment of bone metastases from breast cancer.
    Authors: Buijs JT, Henriquez NV, van Overveld PG, van der Horst G, Que I, Schwaninger R, Rentsch C, ten Dijke P, Cleton-Jansen AM, Driouch K, Lidereau R, Bachelier R, Vukicevic S, Clezardin P, Papapoulos SE, Cecchini MG, Lowik CW, Van Der Pluijm G
    Cancer Res., 2007;67(18):8742-51.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human BMP-7 Quantikine ELISA Kit

Average Rating: 4.7 (Based on 3 Reviews)

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Human BMP-7 Quantikine ELISA Kit
By Anonymous on 06/20/2016
Sample Tested: Cartilage tissue

Human BMP-7 Quantikine ELISA Kit
By Anonymous on 05/09/2016
Sample Tested: Bone Extracts

Very easy to follow instructions.


Human BMP-7 Quantikine ELISA Kit
By Anonymous on 05/04/2016
Sample Tested: demineralized bone extracts