Detects human C1qTNF4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) C1qTNF5 is observed and less than 1% cross-reactivity with rhC1qTNF1 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human C1qTNF4 Leu17-Leu329 Accession # Q9BXJ3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human C1qTNF4
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of C1qTNF4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human C1qTNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3456, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cq1TNF4, also known as CTRP4, is a secreted protein and is one of at least 9 paralogs of Acrp30/Adiponectin. Proteins in this family contain a globular C-terminal domain similar to that of the C1q and TNF ligand family. C1qTNF4 is the only family member that lacks the collagen-like domain but has two C1q domains. Mature human C1qTNF4 shares 92.4% amino acid sequence homology with the corresponding region of mouse C1qTNF4.
C1q And Tumor Necrosis Factor Related Protein 4
Entrez Gene IDs:
114900 (Human); 67445 (Mouse); 311184 (Rat)
C1q and tumor necrosis factor related protein 4; complement C1q tumor necrosis factor-related protein 4; complement-c1q tumor necrosis factor-related protein 4; CTRP4; CTRP4ZACRP4; ZACRP4
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