C1qTNF5 in Human Liver. C1qTNF5 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human C1qTNF5 Monoclonal Antibody (Catalog # MAB3167) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of C1qTNF5 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human C1qTNF5 Monoclonal Antibody (Catalog # MAB3167, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
C1qTNF5, also known as CTRP5, belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF‑related protein family. All family members share a modular organization comprising an N‑terminal signal peptide, a short variable region, a collagenous domain and a C‑terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. Human and mouse C1qTNF5 share 94% amino acid sequence identity.
C1q And Tumor Necrosis Factor Related Protein 5
Entrez Gene IDs:
114902 (Human); 235312 (Mouse)
C1q and tumor necrosis factor related protein 5; Complement C1q Tumor Necrosis Factor-Related Protein 5 Precursor Variant 3; CTRP5; CTRP5LORDDKFZp586B0621; LORD 6; Myonectin 3
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