Detection of Human CaM Kinase IIδ by Western Blot.
Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase IIδ Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase IIδ isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CaM Kinase II delta
Calcium/calmodulin-dependent protein kinase type II delta (CaMKIIδ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.
Calcium/calmodulin-dependent Protein Kinase II delta Subunit
Entrez Gene IDs:
817 (Human); 108058 (Mouse); 24246 (Rat)
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CaM Kinase II delta; CaM kinase II subunit delta; CAMK2D; CAMKD; CaMK-II delta subunit; CaMK-II subunit delta; CaM-kinase II delta chain; DKFZp686G23119; DKFZp686I2288; EC 2.7.11; EC 188.8.131.52; MGC44911
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