Human Cannabinoid R2/CB2/CNR2 Antibody

MAB3655 has been discontinued. View all Cannabinoid R2/CB2/CNR2 products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Cannabinoid R2/CB2/CNR2 in direct ELISAs. In flow cytometry, stains Cannabinoid R2 transfected cells but not irrelevant or Cannabinoid R1 transfectants.
  • Source
    Monoclonal Mouse IgG2A Clone # 352114
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human Cannabinoid R2/CB2/CNR2
    Met1-Cys360
    Accession # NP_001832
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human spleen
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Cannabinoid R2/CB2/CNR2 in HEK293 Human Cell Line Transfected with Human Cannabinoid R2/CB2/CNR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/CNR2 or (B) human Cannabinoid R1/CB1/CNR1 and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Monoclonal Antibody (Catalog # MAB3655) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cannabinoid R2/CB2/CNR2
Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.
  • Long Name:
    Cannabinoid Receptor 2
  • Entrez Gene IDs:
    1269 (Human); 12802 (Mouse); 57302 (Rat)
  • Alternate Names:
    Cannabinoid R2; cannabinoid receptor 2 (macrophage); CannabinoidR2; CB-2; CB2cannabinoid receptor 2; CB2R; CNR2; CX5; CX5testis-dominant CNR2 isoform CB2; hCB2
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