Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

Catalog # Availability Size / Price Qty
MAB3365
MAB3365-SP
Product Details
FAQs
Supplemental Products
Reviews

Human Carbohydrate Sulfotransferase 15/CHST15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 388421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfotransferase 15/CHST15
Ser99-Thr561
Accession # Q7LFX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Carbohydrate Sulfotransferase 15/CHST15 (Catalog # 3365-ST)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 15/CHST15

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST or CHST15) is a type II transmembrane protein composed of a short N‑terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. CHST15 transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A) as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of CHST15 in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human CHST15 was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

References
  1. Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894.
  2. Zou, P. et al. (2003) Glycobiology 13:35.
  3. Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155.
  5. Robbins, P.W. (1962) Methods in Enzymol. 5:964.
  6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  7. Wu, Z.L. et al. (2002) FASEB J. 16:539.
Entrez Gene IDs
51363 (Human); 286974 (Rat)
Alternate Names
B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; BRAG; BRAGKIAA0598RP11-47G11.1; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; Carbohydrate Sulfotransferase 15; CHST15; DKFZp781H1369; EC 2.8.2.33; GALNAC4S6ST; GalNAc4S-6ST; hBRAG; MGC34346; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

There are currently no reviews for this product. Be the first to review Human Carbohydrate Sulfotransferase 15/CHST15 Antibody and earn rewards!

Have you used Human Carbohydrate Sulfotransferase 15/CHST15 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review