Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

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Product Details
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Human Carbohydrate Sulfotransferase 15/CHST15 Antibody Summary

Species Reactivity
Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 388421
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Sulfotransferase 15/CHST15
Accession # Q7LFX5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Carbohydrate Sulfotransferase 15/CHST15 (Catalog # 3365-ST)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 15/CHST15

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST or CHST15) is a type II transmembrane protein composed of a short N‑terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. CHST15 transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A) as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of CHST15 in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human CHST15 was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

  1. Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894.
  2. Zou, P. et al. (2003) Glycobiology 13:35.
  3. Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155.
  5. Robbins, P.W. (1962) Methods in Enzymol. 5:964.
  6. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  7. Wu, Z.L. et al. (2002) FASEB J. 16:539.
Entrez Gene IDs
51363 (Human); 286974 (Rat)
Alternate Names
B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; BRAG; BRAGKIAA0598RP11-47G11.1; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; Carbohydrate Sulfotransferase 15; CHST15; DKFZp781H1369; EC; GALNAC4S6ST; GalNAc4S-6ST; hBRAG; MGC34346; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase

Product Datasheets


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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