Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 303123
View all Carbonic Anhydrase IX/CA9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790

Specificity

Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody

Detection of Carbonic Anhydrase IX/CA9 antibody in U-87 MG Human Cell Line antibody by Flow Cytometry.

Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry.

U-87 MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 APC-conjugated Monoclonal Antibody (Catalog # FAB2188A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: U‑87 MG human glioblastoma/astrocytoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using FAB2188A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

References

  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Gene Symbol

CA9

UniProt

Q16790

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody

Customer Reviews for Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HEK 293 cell line
    Species: Human
    Verified Customer | Posted 04/01/2021
    Green: CA9 transfected cells
    Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody FAB2188A
  • Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: SK-OV-3 human ovarian adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 06/15/2018
    SK-OV3 cells (negative for CA IX expression) and SK-OV3 transfected with human CA IX were stained analyzed by flow cytometry
    Human Carbonic Anhydrase IX/CA9 APC‑conjugated Antibody FAB2188A

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