Human Carbonic Anhydrase IX/CA9 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
  • Source
    Monoclonal Mouse IgG2A Clone # 303123
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
    Accession # Q16790
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 APC‑conjugated Monoclonal Antibody (Catalog # FAB2188A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

  • References:
    1. Pastorek, J. et al. (1994) Oncogene 9:2877.
    2. Opavsky, R. et al. (1996) Genomics 33:480.
    3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
    4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
    5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
  • Entrez Gene IDs:
    768 (Human); 230099 (Mouse)
  • Alternate Names:
    CA9; CA-IX; CAIXcarbonic anhydrase 9; Carbonate dehydratase IX; carbonic anhydrase IXpMW1; carbonic dehydratase; EC; G250; Membrane antigen MN; MN; MNRCC-associated antigen G250; P54/58N; RCC; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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