Human Caspase-14 Antibody

Catalog # Availability Size / Price Qty
MAB8215
MAB8215-SP
Human Caspase-14 Antibody in Data
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Human Caspase-14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspase-14 in direct ELISA and Western Blot.
Source
Monoclonal Mouse IgG2B Clone # 868715
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Caspase-14
Ser2-Gln242
Accession # P31944
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Caspase-14 antibody by Western Blot. View Larger

Detection of Human Caspase-14 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for full length Caspase-14 at approximately 28-30 kDa and the p10 subunit at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Caspase-14 antibody in Human Epidermis by Immunohistochemistry (IHC-P). View Larger

Caspase-14 in Human Epidermis. Caspase-14 was detected in immersion fixed paraffin-embedded sections of human epidermis using Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of suprabasal keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Caspase-14 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Caspase-14 by Simple WesternTM. Simple Western lane view shows lysates of human skin tissue, loaded at 0.5 mg/mL. Specific bands were detected for full length Caspase-14 at approximately 36 kDa and the p10 subunit at approximately 12 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Caspase-14 Monoclonal Antibody (Catalog # MAB8215). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-14

Caspase-14 belongs to the evolutionarily conserved caspase family of cysteinyl aspartate-specific proteinases that frequently play a central role in apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce large and small subunits that dimerize to form an active enzyme. Caspase-14 is processed to form p19 and p10 subunits, but unlike many caspases, this activation is believed to be non-apoptotic. Expressed within hair follicles and sebaceous glands in the epidermis, Caspase-14 processing is implicated in terminal keratinocyte differentiation and cornification. Caspase-14 expression may also protect against psoriasis and epidermal UVB photodamage. Full-length human Caspase-14 is 242 amino acids (aa) in length, and shares 71% and 72% aa sequence identity with mouse and rat Caspase-14, respectively.

Entrez Gene IDs
23581 (Human); 12365 (Mouse); 299587 (Rat)
Alternate Names
apoptosis-related cysteine protease; CASP14; CASP-14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis-related cysteine protease; Caspase14; Caspase-14; EC 3.4.22.-; MGC119078; MGC119079; MICE

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human Caspase-14 Antibody
By Anonymous on 06/17/2019
Application: Simple Western Sample Tested: Skin tissue Species: Human

Detection of caspase-14 by Simple Western (JESS) in reconstructed human epidermis lysate (about 200 µg/mL protein concentration).
Antibody dilution: 1/50